Were cleaned5.five cm diameter, 7 the flowering period persisted. At the grain size, sediment corers (n = two, from fauna cm and sediment withtaken before, throughout and following the sexual reproduction period. Sedi of length) had been artificial seawater (30 psu). Then, the sexual spathes contained in every single corer werewere driedrecording the flowering effort. ment corers counted for for 72 h at 60 to measure the organic matter content from the The reproductive phenology of Z. GNF6702 MedChemExpress noltei sediment (450 , 4.five h). by analysing sediment (OM) by loss on ignition in 1 g ofin Ria de Aveiro was assessedThen, sediment wa the spathes beneath a dissecting microscope and assigning them a stage of development separatedon their morphological traits. In sieve shaker distinctive SIEVING TECHNOL into seven sizes using a mechanical this way, five (CISA# stages of sexual primarily based OGIES BA 200 N; t = defined (Table 1, Figure1.2 mm), and subsequently classified following reproduction were 20 min, amplitude = two). Stages I, II and III corresponded for the the period of flowering formation, gravel (two IV andvery coarse with the period of seed sand Wentworth scale [28]: fine when stages mm), V coincided sand (1 mm), coarse formation and maturation. Broken and necrotic spathes have been quantified as abortions. (0.5 mm), medium sand (0.25.5 mm), fine sand (0.125.25 mm), pretty fine sand (0.063The total variety of spathes collected 0.125 mm) and silt and clay (0.063 mm). in each reproductive stage through the study period was calculated to describe the basic reproductive phenology of Z. noltei inside the Ria de Aveiro lagoon. To test differences amongst meadows within the reproductive phenology, the 2.2. Flowering Work and Reproductive Phenology percentage of spathes in every reproductive stage was estimated by meadow and date.To study the flowering effort plus the reproductive phenology of Z. noltei in the Ria de Aveiro lagoon, seagrass corers (n = 4; 9 cm of diameter, 6.5 cm of length) have been randomly collected at 5 dates (June, July, August, September and November) in every meadow a the flowering period persisted. At the laboratory, seagrass corers have been cleaned from fauna and sediment with artificial seawater (30 psu). Then, the sexual spathes contained in eachPlants 2021, 10, x FOR PEER REVIEW15 ofPlants 2021, ten,Table 1. Description from the 5 sexual reproductive stages used to study the reproductive phenology of Z. noltei in Ria de Aveiro. The morphology of every reproductive stage is shown in Figure 2.four ofTable 1. Description of your five sexual reproductive stages used to study the reproductive phenology of Z. noltei in Ria de Period Stage Description Aveiro. The morphology of each and every reproductive stage is shown in Figure 2. Yellow-green spathe, sheath closed; pistils and stamina are visible, aligned onto I the stem Period Stage Description Pistils (IIa) and/or stamina erected (IIb); designs and stigma and/or anthers are Flowering II Yellow-green spathe, sheath closed; pistils and stamina are visible, aligned onto the stem I outdoors the sheath Flowering II Pistils (IIa) and/or stamina erected (IIb); designs and stigma and/or anthers are outdoors the sheath Stigma brown, start to depart from spathe; often with stamina currently detached III III Stigma brown, start off to depart from spathe; generally with stamina already detached in the spathe from the spathe IV Green spathe with YC-001 Technical Information immature seeds; sheath closed IV Green spathe with immature seeds; sheath closed Seed formation Green/brown spathe, dark brown.
