-violaxanthal, glandulone B, methyl (9Z)-6 -oxooxo-6,5′-diapo-6-carotenoate, and ganoderiol
-violaxanthal, glandulone B, methyl (9Z)-6 -oxooxo-6,5′-diapo-6-carotenoate, and ganoderiol C. six,5 -diapo-6-carotenoate, and ganoderiol C.Figure 5. Venn diagram contemplating the VIP discriminant compounds in accordance with either “origin” or “plantspecies”. “plant species”.Figure 5. Venn diagram contemplating the VIP discriminant compounds in line with either “origin” or3.4. Carotenoid Contents in Pumpkin Pulp Extracts The carotenoid contents inside the pumpkin samples had been analysed by HPLC-DAD, and also the outcomes are reported in Figure six. The data show that there was a sizable variability within the cumulative total carotenoids content material (CC), ranging from 2.09 (sample 22) to 0.04 (sampleAntioxidants 2021, 10,11 of3.four. Carotenoid Contents in Pumpkin Pulp Extracts The carotenoid contents inside the pumpkin samples had been analysed by HPLC-DAD, along with the benefits are reported in Figure 6. The information show that there was a sizable variability inside the cumulative total carotenoids content (CC), ranging from two.09 (sample 22) to 0.04 (sample 384) mg/g dry matter, with much more than half the samples bearing a total cumulative content 0.5 mg/g. The distinct values of CC for each and every access had been probably as a result of genetic influence as an alternative to climate, soil, sowing, harvesting, and storage situations, due to the fact these variables have been exactly the same for all of the samples. The 5 accessions using a CC above 1.0 mg/mL (22, 173, 177, 212, 245) had related contents of -carotene, except for sample 173, which had slightly much less. In general, one particular could possibly anticipate that the pulp of those pumpkins will be highly coloured, considering the fact that –MNITMT In Vitro correlated. The graph also shows huge variability inside the 4 varieties of carotenoids quantified, with sample 22 possessing the highest content material of -carotene and amongst the highest for carotene, but zeaxanthin and lutein had been hardly detected. Probably the most consistent carotenoid detected was -carotene, located in all samples, albeit at distinctive concentrations. Interestingly, zeaxanthin was detected in all pumpkins not originally from Serbia, 12 of 17 except in two (23 and 384), whereas in Serbian pumpkins, this carotenoid was present in only 4 samples (2, three, 4, 212) out on the 13 tested.Antioxidants 2021, ten,Figure six. Total carotenoid content in in pumpkin samples measuredHPLC-DAD. Outcomes are expressed as mg/g dry matter. six. Total carotenoid content material pumpkin samples measured by by HPLC-DAD. Results are expressed as mg/g dry matter. The cumulative carotenoidfor every single sample is sample is broken down into the four carotenoids (lutein, zeaxanthin, The cumulative carotenoid content content for each and every broken down in to the four carotenoids calculated calculated (lutein, zeaxanthin, -carotene). -carotene). Serbian samples are indicated in bold.expressed asexpressed as mean values S.D (n -carotene, -carotene, Serbian samples are indicated in bold. Results are Final results are mean values S.D (n = two). = 2).four. Discussion four. Discussion Prior studies have reported that pumpkins and their by-products show variPrevious studies have reported that pumpkins and their by-pr.