United states of america Dollar; v: Version; Vp: Viviparous.Acknowledgements We Caspase 3 manufacturer gratefully acknowledge the monetary help provided by Alberta Wheat Commission and AAFC Canadian National Wheat Cluster Plan. We tremendously acknowledge the technical assistance offered by Leslie Bihari and Zafrin Akter in production of doubled haploid population, and assist received in phenotyping by Mark Virginallo. We also acknowledge the support of Klaus Strenzke (University of Alberta, Edmonton) and Mark Virginallo, Kelly Ryan, and Kim Ziegler (Lethbridge Analysis and Improvement Centre, Lethbridge) within the field experiments. Authors’ contributions HSR developed the genetic mapping population. RD and HSR made the study. HSR, MES and DS grown the mapping population in Lethbridge, Ithaca and Edmonton, respectively. RD and MES phenotyped the mapping population. CH genotyped the mapping population. RG offered the pedigree facts of AAC Tenacious. RD performed all analyses, interpreted the results and writing from the original draft. All authors read and approved the final manuscript. Authors’ details RD is a Research Biologist and CH, RJG and HSR are Study Scientists at Agriculture and Agri-Food Canada. MES and DS are Professors at Cornell University, USA and University of Alberta, Canada, respectively. JS is definitely an Associate Professor at McGill University, Canada. Funding This research was funded by Alberta Wheat Commission and AAFC Canadian National Wheat Cluster Program. Availability of data and materials Linkage map of AAC Innova/AAC Tenacious doubled haploid mapping population of spring wheat, that is utilized for the present study, has been published previously by authors (Dhariwal et al. [75]; doi.org/10.3390/ ijms21124497). Linkage map is publically readily available as supplementary info with the published paper [75] and may either be downloaded from the link: mdpi/1422-0067/21/12/4497#supplementary or requested in the corresponding author. All other data generated or analysed throughout this study are integrated within this published post and its supplementary details files.Supplementary InformationThe online version contains supplementary material offered at doi. org/10.1186/s12864-021-08209-6. More file 1: Figure S1. Pre-harvest sprouting (PHS) phenotypes of check cultivars soon after four days inside a mist chamber. Spike bundles from left to right of resistant (Cardale, Enchant, Stettler, AAC Tenacious, Penhold, CDC Stanley), intermediate (Vesper, AAC Indus, Carberry, Lillian, AAC Chiffon, AAC Brandon, AAC Foray) and susceptible (Sadash, AAC Innova, AAC Amazing, Conquer, AC Andrew) cultivars are shown in upper, middle and reduced rows, respectively. Extra file 2: Tables S1 to S9. DNA Methyltransferase Species Particulars of pre-harvest sprouting information, quantitative trait loci, connected markers, genetic/genomic positions, and preceding research. More file three: Figure S2. Effects of pre-harvest sprouting (PHS) resistance quantitative trait loci (QTLs) on sprouting. Effects of QTLs QPhs. lrdc-1A.two, QPhs.lrdc-2B.1, QPhs.lrdc-3A.1, QPhs.lrdc-3B.2, QPhs.lrdc-3D.1, QPhs. lrdc-3D.2 and QPhs.lrdc-7D, respectively, shown as 1A.2, 2B.1, 3A.1, 3B.two, 3D.1, 3D.two and 7D, are presented as bar plot and line graph. Additional file four: Figure S3. Complete pedigree of wheat cultivar AAC Tenacious. Unique cultivars/landraces and their crosses in pedigree are shown in circles. Female and male parents are shown by pink and blue line connections. Circle colors dark green, light green, dark red, light red,
