Rabbit anti-BNIP3(BH3 Domain Specific) Polyclonal Antibody

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Product Details FAQ Citations(0) Video Pictures Documents |Overview |Description Purified Rabbit Polyclonal Antibody (Pab) |Synonym BCL2/adenovirus E1B 19 kDa protein-interacting protein 3; BNIP3; NIP3 |Host Rabbit |Specificity This BNIP3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 152-187 amino acids from human BNIP3. |Species Reactivity Human, Mouse |Applications IF~~1:50~100WB~~1:1000IHC-P~~1:50~100 |Properties |Clonality Polyclonal Antibody |Isotype Rabbit Ig |Storage Temp. Maintain refrigerated at 2-8 ° C for up to 2 weeksFor long time storage store at -20 ° C in small aliquots to prevent free that cycles |Storage Buffer Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |Research areas Cancer;Cardiovascular;Cell Biology;Metabolism;Microbiology;Autophagy |Target |Background Apoptosis-inducing protein that can overcome BCL2 suppression. May play a role in repartitioning calcium between the two major intracellular calcium stores in association with BCL2. Involved in mitochondrial quality control via its interaction with SPATA18/MIEAP: in response to mitochondrial damage, participates to mitochondrial protein catabolic process (also named MALM) leading to the degradation of damaged proteins inside mitochondria. The physical interaction of SPATA18/MIEAP, BNIP3 and BNIP3L/NIX at the mitochondrial outer membrane regulates the opening of a pore in the mitochondrial double membrane in order to mediate the translocation of lysosomal proteins from the cytoplasm to the mitochondrial matrix. Plays an important role in the calprotectin (S100A8/A9)-induced cell death pathway. |Cellular localization Mitochondrion. Mitochondrion outer membrane; Single-pass membrane protein. Note=Coexpression with the EIB 19- kDa protein results in a shift in NIP3 localization pattern to the nuclear envelope. Colocalizes with ACAA2 in the mitochondria Colocalizes with SPATA18 at the mitochondrion outer membrane |UniProt Q12983 |References |References References for protein:1.Kothari, S., et al., Oncogene 22(30):4734-4744 (2003). 2.Lee, S.M., et al., Life Sci. 71(19):2267-2277 (2002). 3.Ray, R., et al., J. Biol. Chem. 275(2):1439-1448 (2000). 4.Chen, G., et al., J. Biol. Chem. 274(1):7-10 (1999). 5.Yasuda, M., et al., J. Biol. Chem. 273(20):12415-12421 (1998).References for HepG2 cell line:1. Knowles BB, et al. (1980). Human hepatocellular carcinoma cell lines secrete the major plasma proteins and hepatitis B surface antigen. Science 209: 497-499.[ PubMed: 6248960].2. Darlington GJ, et al. (1987). Growth and hepatospecific gene expression of human hepatoma cells in a defined medium. In Vitro Cell. Dev. Biol. 23: 349-354.[PubMed: 3034851].3. Ihrke, G; Neufeld, EB; Meads, T; Shanks, MR; Cassio, D; Laurent, M; Schroer, TA; Pagano, RE et al. (1993). “WIF-B cells: an in vitro model for studies of hepatocyte polarity”. Journal of Cell Biology 123 (6): 1761–1775. [PubMed:7506266]. 4. Mersch-Sundermann, V.; Knasmüller, S.; Wu, X. J.; Darroudi, F.; Kassie, F. (2004). “Use of a human-derived liver cell line for the detection of cytoprotective, antigenotoxic and cogenotoxic agents”. Toxicology 198 (1–3): 329–340. [PubMed:15138059]. |Tips:This product is for research use only. Not for use in diagnostic prodcedures.