Ntact heart. It is also important to note that isolated myocytes in the current study were not tested under loading conditions. Loading conditions can influence muscle function and altered loading theoretically could impact isolated myocyte shortening and tension development. Unfortunately, the technical difficulty of myocyte loading experiments limits its utility and widespread implementation [37,71]. Unloaded isolated myocyte assessment still is an important tool for evaluating shortening velocity/cross bridge turnover rate and comparisons between treatment groups can be made under similar experimental conditions without influence of potential confounders. Finally, we acknowledge that comprehensive isolated myocyte morphometric analysis would have complemented the echocardiographic and isolated myocyte functional data presented in the current study. Although we are accustomed to high proportions of rod cellAcknowledgmentsWe would like to thank Mrs. April Beyer and Drs James Kuzman and Jinghai Chen for their technical assistance.Author ContributionsConceived and designed the experiments: NYW DW AMG. Performed the experiments: NYW DW RAR. Analyzed the data: NYW DW AMG. Wrote the paper: NYW. Edited and revised manuscript: AMG.
There is increasing interest in literature to understand the olfactory deficits of depression. An overview of this literature shows conflicting results regarding impairment of all olfactory parameters (i.e., odor threshold, odor identification, discrimination, intensity, familiarity and pleasantness). On the one hand, some studies [1?] showed odor identification deficits in major depressive episode (MDE). Atanasova et al. (2010) [4] demonstrated that olfactory impairments (odor intensity, discrimination and odor pleasantness) depended 18055761 on the valence of the stimuli. Regarding odor pleasantness, some research teams showed that depressed patients over-evaluated the pleasantness of odors compared to controls [5,6]. On the other hand, different studies found no significant difference between patients suffering from MDE and healthy controls concerning the odor pleasantness [6?], the odor identification [5,7,10?4] and the evaluation of odor intensity [5,6,9,15]. The inconsistent findings in this field may be explained by differences 
in the methodological approaches (e.g., battery of testing, scoring), the clinical type of depression (e.g., seasonal, unipolar, bipolar) and the inclusion criteria of the participants (e.g., medicated or not, types of medications). For instance, the calculation method of the scores of identification, intensity or pleasantness usually considers all the odors, irrespective of the hedonic valence (or pleasantness) of the stimuli. This method does not allow to emphasize the differences between odorants, while itis of particular importance in MDE as anhedonia is a cardinal symptom of the disease (DSM-IV) [16] and the hedonic valence of a component would influence the patient’s ability to identify an odor and evaluate its intensity and pleasantness. This hypothesis is supported by the strong relationships between clinical and sensory anhedonia in the olfactory [9] and the gustatory fields [17]. For these reasons, it is crucial to investigate odor perception using different single odorants in order to evaluate their specific emotional impact on olfactory capabilities. MedChemExpress Castanospermine Consequently, 
the present study used olfactory stimuli with different hedonic valence, and the scores were calculated CAL120 supplier separately for e.Ntact heart. It is also important to note that isolated myocytes in the current study were not tested under loading conditions. Loading conditions can influence muscle function and altered loading theoretically could impact isolated myocyte shortening and tension development. Unfortunately, the technical difficulty of myocyte loading experiments limits its utility and widespread implementation [37,71]. Unloaded isolated myocyte assessment still is an important tool for evaluating shortening velocity/cross bridge turnover rate and comparisons between treatment groups can be made under similar experimental conditions without influence of potential confounders. Finally, we acknowledge that comprehensive isolated myocyte morphometric analysis would have complemented the echocardiographic and isolated myocyte functional data presented in the current study. Although we are accustomed to high proportions of rod cellAcknowledgmentsWe would like to thank Mrs. April Beyer and Drs James Kuzman and Jinghai Chen for their technical assistance.Author ContributionsConceived and designed the experiments: NYW DW AMG. Performed the experiments: NYW DW RAR. Analyzed the data: NYW DW AMG. Wrote the paper: NYW. Edited and revised manuscript: AMG.
There is increasing interest in literature to understand the olfactory deficits of depression. An overview of this literature shows conflicting results regarding impairment of all olfactory parameters (i.e., odor threshold, odor identification, discrimination, intensity, familiarity and pleasantness). On the one hand, some studies [1?] showed odor identification deficits in major depressive episode (MDE). Atanasova et al. (2010) [4] demonstrated that olfactory impairments (odor intensity, discrimination and odor pleasantness) depended 18055761 on the valence of the stimuli. Regarding odor pleasantness, some research teams showed that depressed patients over-evaluated the pleasantness of odors compared to controls [5,6]. On the other hand, different studies found no significant difference between patients suffering from MDE and healthy controls concerning the odor pleasantness [6?], the odor identification [5,7,10?4] and the evaluation of odor intensity [5,6,9,15]. The inconsistent findings in this field may be explained by differences in the methodological approaches (e.g., battery of testing, scoring), the clinical type of depression (e.g., seasonal, unipolar, bipolar) and the inclusion criteria of the participants (e.g., medicated or not, types of medications). For instance, the calculation method of the scores of identification, intensity or pleasantness usually considers all the odors, irrespective of the hedonic valence (or pleasantness) of the stimuli. This method does not allow to emphasize the differences between odorants, while itis of particular importance in MDE as anhedonia is a cardinal symptom of the disease (DSM-IV) [16] and the hedonic valence of a component would influence the patient’s ability to identify an odor and evaluate its intensity and pleasantness. This hypothesis is supported by the strong relationships between clinical and sensory anhedonia in the olfactory [9] and the gustatory fields [17]. For these reasons, it is crucial to investigate odor perception using different single odorants in order to evaluate their specific emotional impact on olfactory capabilities. Consequently, the present study used olfactory stimuli with different hedonic valence, and the scores were calculated separately for e.