To assess MHC class II-limited processing, LPS and CyaA-handled DCs had been incubated with a mixture of OVA-Alexa647, employed as a marker for Ag uptake, and OVA labeled with BODIPYHFL dye (OVA-DQ)
CD4+ T cells have been gathered and stained with CD4-PerCP (BD Biosciences), CD25-APC and...