With the QUACPAC program of OpenEye software [45], and ROSETTA ligand params files generated with the provided molfile_to_params python script as included in the 3.3 distribution. No Methionine enkephalin catalytic constraints were used for the enzyme design application runs, effectively making it a receptor design application. 1000 designs were created for every protein and every mutation on that protein with experimental affinity data in the test set. The best design was determined by the Docosahexaenoyl ethanolamide custom synthesis ranking scheme suggested in the documentaComputational Design of Binding Pocketstion, it is the design with the best predicted binding energy among the designs with the 10 top total scores.Author ContributionsConceived and designed the experiments: CM OK BH. Performed the experiments: CM JK. Analyzed the data: CM OK BH. Contributed reagents/materials/analysis tools: MS NT. Wrote the paper: CM BH.Supporting InformationInformation S(PDF)
Since they were first described, microRNAs (miRNAs) have been studied widely for their role in the regulation of gene expression [1,2,3,4,5]. MiRNAs are best known for the ability to down-regulate protein expression by directly or indirectly inhibiting transcription or by degrading mRNA transcripts [1,4,5,6,7,8]. But they can also activate translation under certain environmental conditions [5]. MiRNAs are usually transcribed from intergenic regions or the antisense strands of genes [9,10]. However, significant numbers of miRNAs have been discovered in introns and even exons of protein encoding genes [10]. Precursor miRNAs undergo extensive enzyme-mediated processing which results in a single-stranded molecule that is approximately 22 nucleotides in length. In the human genome, more than 1,500 mature miRNA transcripts have been characterized thus far [11]. Functionally, miRNAs can target mRNA molecules involved in many biological processes, including cell growth and development, cell fate, and apoptosis [12,13,14]. Given that miRNA transcripts affect nearly every aspect of cellular function, it is not surprising that they play a critical role in the etiology of a wide variety ofdisease manifestations [15]. Indeed, miRNAs have been implicated in many types of cancers, as well as specific cardiac and neurologic diseases [16,17,18,19,20,21,22,23]. 24195657 Furthermore, studies have identified tissue-specific miRNA signatures that have the potential to act as diagnostic markers in human disease [19,24,25]. For this reason, it is critical that methods for detection and quantification of miRNAs in a clinical setting are sufficiently sensitive and specific in order to distinguish healthy and disease states. Research studies have characterized several different platforms for miRNA expression profiling by assaying synthetic RNA or RNA from commercially available cell lines and tissues [26,27,28,29]. Others have described the detection and quantification of miRNA transcripts in samples from both fresh frozen (FF) and formalin-fixed paraffin-embedded (FFPE) tissues from human patients [30,31]. These studies have highlighted the great diversity of methods that are available for miRNA expression analysis. Notably, these technologies exhibit different dynamic ranges and resolution capabilities, making it difficult to determine true miRNA expression levels.Multi-Platform Analysis of MicroRNA ExpressionGene expression microarrays are relatively inexpensive and are useful for profiling the miRNA transcriptome in a single experiment. However, studies have shown signif.With the QUACPAC program of OpenEye software [45], and ROSETTA ligand params files generated with the provided molfile_to_params python script as included in the 3.3 distribution. No catalytic constraints were used for the enzyme design application runs, effectively making it a receptor design application. 1000 designs were created for every protein and every mutation on that protein with experimental affinity data in the test set. The best design was determined by the ranking scheme suggested in the documentaComputational Design of Binding Pocketstion, it is the design with the best predicted binding energy among the designs with the 10 top total scores.Author ContributionsConceived and designed the experiments: CM OK BH. Performed the experiments: CM JK. Analyzed the data: CM OK BH. Contributed reagents/materials/analysis tools: MS NT. Wrote the paper: CM BH.Supporting InformationInformation S(PDF)
Since they were first described, microRNAs (miRNAs) have been studied widely for their role in the regulation of gene expression [1,2,3,4,5]. MiRNAs are best known for the ability to down-regulate protein expression by directly or indirectly inhibiting transcription or by degrading mRNA transcripts [1,4,5,6,7,8]. But they can also activate translation under certain environmental conditions [5]. MiRNAs are usually transcribed from intergenic regions or the antisense strands of genes [9,10]. However, significant numbers of miRNAs have been discovered in introns and even exons of protein encoding genes [10]. Precursor miRNAs undergo extensive enzyme-mediated processing which results in a single-stranded molecule that is approximately 22 nucleotides in length. In the human genome, more than 1,500 mature miRNA transcripts have been characterized thus far [11]. Functionally, miRNAs can target mRNA molecules involved in many biological processes, including cell growth and development, cell fate, and apoptosis [12,13,14]. Given that miRNA transcripts affect nearly every aspect of cellular function, it is not surprising that they play a critical role in the etiology of a wide variety ofdisease manifestations [15]. Indeed, miRNAs have been implicated in many types of cancers, as well as specific cardiac and neurologic diseases [16,17,18,19,20,21,22,23]. 24195657 Furthermore, studies have identified tissue-specific miRNA signatures that have the potential to act as diagnostic markers in human disease [19,24,25]. For this reason, it is critical that methods for detection and quantification of miRNAs in a clinical setting are sufficiently sensitive and specific in order to distinguish healthy and disease states. Research studies have characterized several different platforms for miRNA expression profiling by assaying synthetic RNA or RNA from commercially available cell lines and tissues [26,27,28,29]. Others have described the detection and quantification of miRNA transcripts in samples from both fresh frozen (FF) and formalin-fixed paraffin-embedded (FFPE) tissues from human patients [30,31]. These studies have highlighted the great diversity of methods that are available for miRNA expression analysis. Notably, these technologies exhibit different dynamic ranges and resolution capabilities, making it difficult to determine true miRNA expression levels.Multi-Platform Analysis of MicroRNA ExpressionGene expression microarrays are relatively inexpensive and are useful for profiling the miRNA transcriptome in a single experiment. However, studies have shown signif.
