Mitting many sclerosis modelCarmen Marin-Ba sco, Margarita Suard z Garc , Issac Hurtado Guerrero, Rafael Maldonado S chez, Guillermo Estivill-Torr , Laura Leyva Fern dez and Oscar Fern dez Fern dezAbstractIntroduction: Mesenchymal stem cells (MSCs) are a multipotent population of adult stem cells, which could represent a promising therapeutic strategy for neurological autoimmune diseases including numerous sclerosis. The mouse is the most used species for acquiring and studying the characteristics of MSC and their prospective as autologous Epipinoresinol methyl ether transplants in pre-clinical models. Nonetheless, conflicting information have already been published disclosing intraspecies variations. The selection of your mouse strain and also the tissue source seem, amongst other folks, as crucial things within the experimental application of MSCs. Solutions: Adipose tissue-derived MSCs obtained from the SJLJCrl mouse strain (SJL-AdMSC) happen to be cultured for any long time (from passage 0 as much as 15) below controlled experimental circumstances, and their growth rate, morphology, stromal and haematopoietic marker expression profiles and differentiation capacity towards adipocytes, osteocytes and chondrocytes happen to be determined. Moreover, their preclinical efficacy has been assessed by autologous transplant in relapsing-remitting experimental autoimmune encephalomielitis (RR-EAE)-induced SJL mice (a well established mice model for the study of RR-multiple sclerosis). Results: We demonstrate that SJL-AdMSCs show the exact same fibroblastic shape, growth rate, profile of markers expression and multipotency described for MSCs in each passage evaluated (up to passage 15). Furthermore, SJL-AdMSCs ameliorate the RR-EAE course, suggesting that they could modulate PubMed ID:http://www.ncbi.nlm.nih.gov/pubmed/21302868 disease progression. Additionally, their functions studied are completely comparable with all the standardized Ad-MSCs obtained in the C57BL6 mouse strain, which strengthens their use in cell therapy. Conclusion: SJL-AdMSCs may be a appropriate supply of Ad-MSCs for studies associated for the properties of MSCs and their application as promising therapeutic tools in autologous transplants in experimental medicine.Introduction Mesenchymal stem cells (MSCs) are a multipotent and heterogeneous subset of adult stromal stem cells present in several tissues. Traditionally, the study in the therapeutic potential of those cells has been oriented to their application in tissue repair and regeneration, on account of their differentiation capacity and transdifferentiation into distinctive cell lines [1,2]. Nevertheless, over the past decade, Correspondence: marga.suardiazgmail.com Equal contributors Unidad de Gesti Cl ica Inter-centros de Neurociencias, Laboratorio de Investigaci y Servicio de Neurolog . Instituto de Biomedicina de M aga (IBIMA), Hospitales Universitarios Regional de M aga y Virgen de la Victoria, 29009 M aga, SpainMSCs happen to be shown to possess a broad spectrum of immunoregulatory capabilities affecting both adaptive and innate immunity. This has been proven by in vitro experiments and their effectiveness when transplanted into animal models of autoimmune ailments [3-5]. MSCs for that reason represent a promising tool as cell therapy, not just for regenerative medicine but additionally for modulation with the immune program [6-9]. The biological characteristics with which cell populations need to comply to become defined as MSCs are clear, regardless of the tissue of origin [10,11]. In recent years, a number of studies have described isolation protocols, culture, expansion, phenotypic and functional characte.
