Ontrol (red and blue lines) lignosulfonates have been very equivalent (Extra file 1: Figure S4), plus the exact same was observed in the 2D-NMR spectra (Extra file 1: Figure S5 cf, ad, respectively). The above results indicate that the phenolic lignin moiety: (i) promoted lignosulfonate modification beneath steady-state conditions; and (ii) was also accountable for the (modest) modification of native lignin by the W164S variant.S zJim ez et al. Biotechnol Biofuels (2016) 9:Page 6 ofaMeO A A A A CB C ACbMeO A ACcMeO A A AB C ACC AAA CC ABB100GGGGGGG7 six 5 4GG6 5 4HeH CfH CdMeO A A(S) A(S) A(G) A A(G) C A(S) A(G) A(S)C CMeO A A(S) A(S) A(G) A(S) C A(G) C A(G) A(S)MeO A A(S) A(G) A A(S) A(G) A(G) C A(S) A(G) A(S)CA(G)A(G)CCS’2,six GS2,six G5 S2,S’2,S2,6 S2,S’2,6 GS2,6 S2,six GG2 G120G7 6 five 4GG6 5 4HHHgHO3SOMe O HO MeOHO MeOOHO (OMe)O(OMe) (MeO) O OMe (MeO)HOOMe O (MeO)O(OMe)SO3HSO3HOHROO O(MeO) OOMeOMeMeO OOMeMeO OOMeMeO OOMeO O OMe OMeAABCGSSSS zJim ez et al. Biotechnol Biofuels (2016) 9:Page 7 of(See figure on preceding page.) Fig. 4 HSQC NMR spectra of softwood (a ) and hardwood (d ) lignosulfonates treated for 24 h with native VP (b, e) and its W164S variant (c, f) and control with no enzyme (a, d), and formulae in the principal structures identified (g). Signals correspond to 13C1H correlations at the diverse positions of lignin nativeoxidizedsulfonated syringyl (red signals) and guaiacyl (green signals) units, Sulfaquinoxaline Biological Activity sulfonatednonsulfonated side chains in O4 (blue signals), phenylcoumaran (cyan signals), and resinol (purple signals) substructures, and methoxyls (orange signal) (gray, unassigned signals). Identical volume of sample (40 mg prior to enzymatic remedy) and DMSOd6 (0.75 mL) have been made use of for all the spectra, which were regular ized for the similar intensity of the DMSO signal (not shown) for comparison. List of signals (CH ppm): 53.23.46, CH in phenylcoumarans (B); 53.43.00, CH in resinols (C); 55.53.66, CH in methoxyls (MeO); 59.43.4 and 3.72, CH in (A); 61.14.00, CH in sulfonated (A); 65.63.93, CH in sulfonated linked to a Gunit (A(G)); 67.24.02, CH in sulfonated linked to a Sunit (A(S)); 70.84.16 and three.77, CH in resinols (C); 71.14.72, CH in linked to a Gunit (A(G)); 71.54.85, CH in linked to a Sunit (A(S)); 79.34.91, C H in sulfonated linked to a G unit (A(G)); 80.94.67, CH in sulfonated linked to a S unit (A(S)); 83.34.24, CH in linked to a G unit (A(G)); 84.94.59, CH in resinols (C); 85.74.08, CH in linked to a S unit (A(S)); 86.75.41, CH in phenylcoumarans (B); 103.86.68, C2H2 and C6H6 in syringyl units (S2,6); 106.27.29, C2H2 and C6H6 in oxidized syringyl units (S’2,6); 108.06.68, C2H2 and C6H6 in sul fonated syringyl units (S2,six); 114.06.60 and 114.36.87, C2H2 and C5H5 in guaiacyl units (G2G5); and 122.86.75, C6H6 in guaiacyl units (G6) (minor, and largely overlapping, signals of C2H2, C5H5 and C6H6 correlations in nonsulfonated guaiacyl units would appear at 110.76.93, 114.26.65 and 118.66.79 ppm, respectively; not shown). Three more ADAM Peptides Inhibitors MedChemExpress aromatic signals within the treated samples, at 126.17.14, 127.77.21 and 128.97.22 ppm, had been assigned to protein (phenylalanine residues within the added enzyme)Comparison with P. chrysosporium LiPThe transient state kinetic constants for reduction of P. chrysosporium LiP CII (the rate-limiting step in catalytic cycle) by native and methylated (nonphenolic) softwood and hardwood lignosulfonates had been obtained (Added file 1: Figure S6). Only the hardwood lignosulfonate (blue continuous line) show.
