L data in Table S2).Values 8000 g/LFreshwater species Marine/estuarine
L data in Table S2).Values 8000 g/LFreshwater species Marine/estuarine species Freshwater species Marine/estuarine speciesValues 8000 g/LFi Aq sh Fi u Aq sha tic ua tic Pl an Pl t an tEC50 – Ag-NPs (g/L) EC50 – Ag-NPs (g/L)5000 4000 4000 3000 3000 2000 2000 1000 1000Mortality/Immobilization Growth/Reproduction Mortality/Immobilization Morphological Growth/Reproduction Physiological Morphological Spatial avoidance Physiological Spatial avoidanceFi shoa lg oa ae lg aeneocM ic rFi shua tic Aq Aqla dAnneocBiological groupsCFigure three. Sensitivity profile for biological groups exposed groups according to the EC50 values. The effects, PHA-543613 supplier represented Biological to Ag-NPs Figure three. Sensitivity profile for biological groups exposed to Ag-NPs depending on the EC50 values. The by distinctive symbols, have been classified based on Table 1. Information in blue and green represent, respectively, freshwater and effects, represented by different symbols, have been classified as outlined by Table 1. Data in blue and estuarine/marine species. Data shown inside the red zone represent results whose EC50 values are larger than 8000 mg/L; Figure 3. Sensitivity profile freshwater and estuarine/marine species. Information on the EC50 values. The green represent, respectively, for biological groups exposed to Ag-NPs basedshown in the red zone therefore, the scale really should not be considered in this zone (see genuine data in Table S3).effects, benefits whose distinctive symbols, have been classified as outlined by Table 1. Information in not and representrepresented by EC50 values are greater than 8000 mg/L; for that reason, the scale shouldblue be green represent, respectively, information in Table S3). GYY4137 manufacturer regarded as within this zone (see realfreshwater and estuarine/marine species. Information shown inside the red zone represent benefits whose EC50 values are larger than 8000 mg/L; thus, the scale ought to not be regarded in this zone (see real information in Table S3).M ic rua ticla dAnpl an tlid sCerapl an tlid seraToxics 2021, 9,7 ofGrowth/reproduction inhibition was the second most typical response and was observed in 12 out of 19 biological groups. For this endpoint, microalgae and copepods were established to become the most sensitive groups; however, microalgae presented the greatest variety of sensitive species with EC50 values decrease than 10 /L: e.g., Isochrysis aff. galbana clone T-ISO (EC50 of 0.4 /L), Cylindrotheca closterium (EC50 of 4.7 /L), Selenastrum capricornutum (EC50 of 6 /L) Chlorella sp. (EC50 of 6 /L) Phaeodactylum tricornutum (EC50 of 9 /L), Chlorella autotrophica (EC50 of 9.six /L). The gastropods Nassarius dorsatus (EC50 of 4.7 /L) and Haliotis rubra (EC50 of 7.1 /L) presented a higher sensitivity to copper when growth/reproduction and morphological alterations had been viewed as as the endpoints, respectively. The sensitivity with the rotifers to copper was represented by lots of distinctive responses, but with ample variation regarding the sensitivity. While the responses related to biochemical alterations, feeding, behavior, and mortality/immobilization were fairly sensitive, the data in the sensitivity profile presented an incredible dispersion regarding the EC50 values. Some species of cnidarian and bivalves seem to be extremely sensitive to copper (EC50 decrease than ten /L; see detail in Table S1). Concerning avoidance, outcomes were obtained for cladocerans, shrimps, amphibians, and fish. In general, the sensitivity on the organisms to avoiding copper is comparable towards the most sensitive values observed within the other biological groups (Figure 1). The mo.