Epithelial cell line WB-F344. Nonetheless, such data, which could be hugely relevant for additional prioritization of in vitro assays appropriate to address the GJIC hallmark inside the IATA for NGTxC, has yet to be systematically mapped and summarized. Thus, this assessment offers a short overview of (1) the role of GJIC in maintaining tissue Homeostasis and biological-mechanistic hyperlinks to cancer/tumor promotion, (2) cell lines and approaches suitable for in vitro GJIC assessment and, lastly, and (three) the results of a systematic search with the application on the SL-DT assay to IFN-lambda 3/IL-28B Proteins Source evaluate GJIC just after the exposure to chemical compounds in a WB-F344 cell line. These in vitro information obtained from the systematic search are compared to IARC, IL-17RD Proteins Biological Activity CompTox/ToxRefDB and Oncologic classification of carcinogens, plus the results (i.e., the SL-DT assay sensitivity,Int. J. Mol. Sci. 2021, 22, x FOR PEER REVIEW4 ofInt. J. Mol. Sci. 2021, 22,four ofobtained in the systematic search are compared to IARC, CompTox/ToxRefDB and Oncologic classification of carcinogens, plus the results (i.e., the SL-DT assay sensitivity, specificity and accuracy) are then discussed regarding the assay utility and its eventual furspecificity and accuracy) are then discussed regarding the assay utility and its eventual ther development for identification, characterization and safety assessment of NGTxC. additional development for identification, characterization and safety assessment of NGTxC. 2. GJIC as the Essential Mechanism in Tissue Homeostasis two. GJIC because the Essential Mechanism in Tissue Homeostasis GJIC is facilitated by gap junctions, plaque-like protein structures that form contiguous GJIC is facilitated by gap junctions, plaque-like protein structures that type contiguous channels betweencells.cells. Vertebratejunctions are constructed from connexins (Cxs), which channels amongst the the Vertebrate gap gap junctions are built from connexins (Cxs), that are membrane proteinsa tetraspan topology of 4 interspersed transmembrane are membrane proteins with using a tetraspan topology of 4 interspersed transmembrane domains connecting the cytoplasmic N-terminal region an extracellular (E1), cytodomains connecting the cytoplasmic N-terminal area via by means of an extracellular (E1), cytoplasmic and yet another extracellularto theloop to the C-terminal Cx molecule [23,26] plasmic and a different extracellular (E2) loop (E2) C-terminal aspect from the aspect of your Cx molecule [23,26] (Figure 1). This structure is shared rodent or 21 20 rodent or 21 human Cx (Figure 1). This structure is shared among the 20 among the human Cx species encoded speciesfamily of Gj/GJ genes. As well as the gene names, the gene names, ofnomenclaby the encoded by the family of Gj/GJ genes. Along with a nomenclature a Cxs primarily based ture of molecular weight predicted by DNApredicted byis also commonly utilized. For exon the Cxs according to the molecular weight sequencing DNA sequencing can also be typically made use of. As an example, Cx43 using a predicted molecular weight ofmolecular weight by ample, Cx43 denotes connexins denotes connexins with a predicted 43 kDa, encoded of 43 kDa, encodedgenes Gja1/GJA1 [23]. InGja1/GJA1 [23]. In gap junctionprotein units are rodent/human by rodent/human genes gap junction channels, six Cx channels, six Cx protein units are organized into a hexameric hemichannel structure termed connexon. organized into a hexameric hemichannel structure termed connexon.Figure 1. Connexins, connexin hemichannels and gap junction channels. A co.
