Of stimulation (Fig. S3), except for TNF-a, where also monocytes are
Of stimulation (Fig. S3), except for TNF-a, where also monocytes are most likely to contribute to the TNF-a detected.—————————————————————————— “Figure three. RhuDex1 impairs cytokine release of WO-LP and PB T cells. Cytokine concentrations were measured in culture supernatants collected following 24 h of stimulation of WO-LPL, or LPS-activated PBMO co-cultured with non-adherent PBL. RhuDex1 and Abatacept were added at the beginning of culture. The mean cytokine responses of every donor within the presence of inhibitors were normalized to the responses without the need of inhibitors (medium, set to one hundred ). For (A) IL-17, (B) IFN-g, (C) IL-2, and (D) TNF-a, the upper graph of each panel depicts responses in WO-LPL (five donors, numbered 1). The decrease graph indicates responses in PBL of 4 allogeneic (allo, numbered I V) and three donors autologous (auto) to WO-LPL. Data points for every single donor are shown in person colors, as well as the mean SD of all data points in each condition is shown as columns and error bars. P 0.05; P 0.01; P 0.001; P 0.0001. Med, medium manage, Aba, Abatacept, Rhu, RhuDex1, inhibitor concentrations in mgmL.2014 The Authors. Immunity, Inflammation and Illness Published by John Wiley Sons Ltd.CD80 Blockage by RhuDex1 Reduces Intestinal T Cell ActivationA.-K. Heninger et al.Acytokine secretion [ ]250 200 150 100 50 0 300IL-17 WO-LPL1 two three 4IL-17 PBLI allo II allo III allo IV allo two auto three autocytokine secretion [ ]200 150 one hundred 50 0 Med Aba 10 Aba 1 Rhu 20 Rhu three Rhu 0.five CD3 Med Aba ten Aba 1 Rhu 20 Rhu 3 Rhu 0.five CD4 auto five autoBcytokine secretion [ ]IFN- WO-LPL0IFN- PBLcytokine secretion [ ]0 Med Aba ten Aba 1 Rhu 20 Rhu 3 Rhu 0.five CDFigure three. Continued.Med Aba ten Aba 1 Rhu 20 Rhu three Rhu 0.5 CD2014 The Authors. Immunity, Inflammation and Disease Published by John Wiley Sons Ltd.A.-K. Heninger et al.CD80 Blockage by RhuDex1 Reduces Intestinal T Cell ActivationCcytokine secretion [ ]250 200 150IL-2 WO-LPL1 2 3 450 0IL-2 PBLI allo II allo III allocytokine secretion [ ]IV allo two auto 3 auto4 auto 5 auto0 Med Aba 10 Aba 1 Rhu 20 Rhu three Rhu 0.5 Med Aba 10 Aba 1 Rhu 20 Rhu 3 Rhu 0.5 CD2 CD3Dcytokine secretion [ ]TNF- WO-LPL0 250 200 150 100 50 0 Med Aba 10 Aba 1 Rhu 20 Rhu three Rhu 0.5 CDFigure three. Continued.2014 The Authors. Immunity, Inflammation and Illness Published by John Wiley Sons Ltd.TNF- PBLcytokine secretion [ ]Med Aba 10 Aba 1 Rhu 20 Rhu 3 Rhu 0.five CDCD80 Blockage by RhuDex1 Reduces Intestinal T Cell ActivationA.-K. Heninger et al.Figure 4. Intracellular cytokine JAK3 review expression of CD4or CD8WO-LP and PB T cells right after activation. (A) Proportion ( ) of CD4and CD8T cells KDM4 MedChemExpress amongst CD3T cells in PBL (three allogeneic donors) and WO-LPL (2 tissue donors). (B) Representative dot-plots (donor III) displaying the intracellular cytokine expression (IL-17, IL-2, IFN-g and TNF-a) of CD4WO-LP T cells (left panel) and CD8WO-LP T cells (correct panel), or (C) of CD4PB T cells (left panel) and CD8PB T cells (ideal panel), as detected within the absence of stimulation, or six h of anti-CD3, or anti-CD2 stimulation.2014 The Authors. Immunity, Inflammation and Illness Published by John Wiley Sons Ltd.A.-K. Heninger et al.CD80 Blockage by RhuDex1 Reduces Intestinal T Cell ActivationAcytokine expression [ ]CD4 WO-LP T cells150 100 50 0 150 100 50 0 150 100 50 0 150 100 50 0 TNFIFNIL-2 IL-Bcytokine expression [ ]CD4 PB T cells150 100 50 0 150 one hundred 50 0 150 100 50 0 150 100 50 0 TNFIFNIL-2 IL-cytokine expression [ ]cytokine expression [ ]cytok.
